Probes for improved melt discrimination and multiplexing in nucleic acid assays
US10975419B2 · kind B2 · utility
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6References
14Claims
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Key dates
| Filing date | Apr 25, 2018 |
| Grant date | Apr 13, 2021 |
| Priority date | — |
| Expiry date | Apr 12, 2039 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6823
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of cleavable probes that comprise a ribonucleotide position that is susceptible to endoribonuclease (e.g., RNase H) cleavage in the presence of target nucleic acid molecules. Probes of the embodiments may also comprise non-natural nucleotide linked to a reporter and/or quenching moiety.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.