Process for cognate nucleotide detection in a nucleic acid sequencing workflow
US10975427B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 17, 2018 |
| Grant date | Apr 13, 2021 |
| Priority date | — |
| Expiry date | Aug 9, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/1241
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Method and composition for identifying cognate nucleotides in a Sequencing By Binding™ procedure, wherein one or more labeled nucleotides are detected in ternary complexes but never incorporated. Labeled nucleotides can be incorporable nucleotides that contact preformed blocked primed template nucleic acids. Alternatively, labeled nucleotides are labeled non-incorporable nucleotides. Labeled nucleotides, including labeled non-incorporable nucleotides, can be detected in ternary complexes in the same reaction mixture that incorporates a reversible terminator nucleotide to create a blocked primed template nucleic acid. Detection of ternary complexes can take place in the presence of a catalytic metal ion.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.