Experimentally validated sets of gene specific primers for use in multiplex applications
US10975440B2 · kind B2 · utility
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13Claims
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Key dates
| Filing date | Apr 19, 2016 |
| Grant date | Apr 13, 2021 |
| Priority date | — |
| Expiry date | Jan 12, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/16
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Sets of experimentally validated gene specific primer pairs are provided. Embodiments of the sets include 10 or more gene specific primer pairs of forward and reverse primers. The forward and reverse primers of each primer pair include gene specific primers that are experimentally validated as suitable for use in a multiplex amplification assay. In some instances, each of the forward and reverse primers includes an anchor domain that includes a universal primer binding site. The sets find use in a variety of different applications, including high-throughput sequencing applications.
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