Read through of truncated proteins in premature termination codon diseases by suppressor tRNAs
US10982209B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 3, 2017 |
| Grant date | Apr 20, 2021 |
| Priority date | — |
| Expiry date | Oct 8, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/10
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Provided are a method for constructing a suppressor tRNA, and 19 suppressor tRNAs corresponding to three termination codons, a plasmid, a vector or a kit comprising the above-mentioned tRNA. Also provided are use of the above-mentioned tRNA, plasmid, vector or kit in the manufacture of a medicament for treating a hereditary disease or a cancer caused by a nonsense mutation of a gene. Also provided are a method for evaluating the efficiency of a suppressor tRNA for reading through a nonsense mutation, and a method for restoring the expression of a truncated protein of a nonsense mutant of a pathogenic gene in a monogenic hereditary disease and a tumor suppressor gene in a tumor cell.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.