Luminophore-labeled molecules coupled with particles for microarray-based assays
US10982267B2 · kind B2 · utility
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Inventors
Key dates
| Filing date | Nov 20, 2018 |
| Grant date | Apr 20, 2021 |
| Priority date | — |
| Expiry date | Feb 19, 2039 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/16
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for labeling target molecules coupled to particles for the detection of the target molecules using a microarray chip, comprises: providing a functionalized microparticle, wherein the microparticle is coated with one or more functional group; providing a modification group on each of the target molecules to be detected to form modified target molecules; contacting the functionalized microparticle with the modified target molecules; coupling a luminophore to the complex between the functionalized microparticle and the modified target molecules, thereby directly or indirectly labeling each modified target molecules with the luminophore. By directly or indirectly labeling the target molecules with the luminophore, the method reduces the cost of fluorescence detection, and avoids PCR inhibition derived from traditional fluorescence labeling molecules.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.