Multipart reagents having increased avidity for polymerase binding

US10982280B2 · kind B2 · utility

Assignee

• Element Biosciences, Inc. · US

Inventors

• Sinan ARSLAN · San Diego, US
• Chunhong Zhou · Pasadena, US
• Molly He · San Diego, US
• Matthew KELLINGER · San Diego, US
• Adeline Huizhen MAH · San Diego, US
• Michael Previte · San Diego, US
• Lei Sun · Santa Clara, US

Key dates

• Filing date: Apr 22, 2020
• Grant date: Apr 20, 2021
• Expiry date: Apr 22, 2040

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2021/6439
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.