Purification of recombinant EV71 virus-like particle and method for preparing vaccine thereof
US10987416B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 28, 2016 |
| Grant date | Apr 27, 2021 |
| Priority date | — |
| Expiry date | Dec 28, 2036 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2030/027
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention provides a method for purifying an EV71 virus-like particle and a method for preparing a vaccine thereof. The virus-like particle is obtained by performing high density fermentation cultivation on recombinantly engineered bacteria; inducing expression of the EV71 virus-like particle protein expression using methanol; collecting the bacteria by centrifugation and performing high-pressure homogenization for disruption; performing precipitation on the supernatant with ammonium sulfate; and purifying by redissolution, ultrafiltration, ion exchange chromatography, molecular sieve chromatography, hydroxyapatite chromatography, etc.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.