Methods of depleting a target molecule from an initial collection of nucleic acids, and compositions and kits for practicing the same

US10988796B2 · kind B2 · utility

Assignee

• Takara Bio USA, Inc. · US

Inventors

• Andrew Alan Farmer · St. Petersburg, US
• Craig Betts · Menlo Park, US
• Nathalie Bolduc · Castro Valley, US

Key dates

• Filing date: Oct 8, 2018
• Grant date: Apr 27, 2021
• Expiry date: Apr 3, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/1003
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided are methods of depleting a target nucleic acid from an initial collection of nucleic acids. Aspects of the methods include contacting the initial collection with a nucleic acid guided nuclease specific for the target nucleic acid in a manner sufficient to deplete the target nucleic acid from the initial collection. Depending on a given application, depletion of a target nucleic acid may vary, e.g., where depleting may include cleaving a target nucleic acid in, or selectively separating a target nucleic acid from, the initial collection of nucleic acids. Also provided are compositions and kits for practicing embodiments of the methods.