Uniform and scalable light-sheets generated by extended focusing
US10989661B2 · kind B2 · utility
2Cited by
38References
20Claims
0Family size
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Key dates
| Filing date | Apr 26, 2016 |
| Grant date | Apr 27, 2021 |
| Priority date | — |
| Expiry date | Apr 26, 2036 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2201/105
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Light-sheet fluorescence microscopy (LSFM) affords highly parallelized 3D imaging with optical sectioning capability and minimal light exposure. However, using Gaussian beams for light-sheet generation results in a trade-off between beam waist thickness and the area over which the beam can approximate a light-sheet. Novel techniques for LSFM are disclosed that uses extended focusing and/or laser line focuses to produce divergence free light-sheets with near diffraction-limited resolution and uniform intensity distribution.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.