Rapid antimicrobial susceptibility testing based on a unique spectral intensity ratio analysis via single fluorescence membrane dye staining and flow cytometry

US10995357B2 · kind B2 · utility

Assignee

• POCARED Diagnostics LTD. · IL

Inventors

• Gal Ingber · Rekhasim, IL
• Moshe Ben-David · Nes Ziona, IL
• Mushki Fridman · Kiryat Malachi, IL
• Yael Gluckman · Rehovot, IL
• Dina Gohman · Holon, IL
• Orly Hammer Munz · Nes Ziona, IL
• Anita Shinderman · Ashdod, IL
• Eran Zahavy · Rehovot, IL

Key dates

• Filing date: Jan 9, 2018
• Grant date: May 4, 2021
• Expiry date: Feb 17, 2038

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2800/52
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Single dye fluorescent staining and the combination of differences in both intensity and spectral emission permit determination of the minimum concentration of an antibiotic needed to inactivate bacteria (Minimum Inhibitory Concentration (MIC)), thereby providing a means for rapid Antibiotic Susceptibility Testing (AST). This allows for a quick and easy means for clinicians to determine a suitable treatment regimen for patients suffering from bacterial infections and those that eventually lead to sepsis.