Methods for generating bispecific shark variable antibody domains and use thereof

US11001640B2 · kind B2 · utility

Assignee

• Merck Patent GmbH · DE

Inventors

• Stefan Becker · Darmstadt, DE
• Bjoern Hock · Maintal, DE
• Stefan Zielonka · Darmstadt, DE
• Harald Kolmar · Göttingen, DE
• Martin Empting · Mühlheim am Main, DE

Key dates

• Filing date: Oct 21, 2015
• Grant date: May 11, 2021
• Expiry date: Aug 30, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/30
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present invention provides a method for generating bispecific shark variable antibody domains (vNAR domains) and uses thereof. The present invention further provides fusion proteins comprising the inventive bispecific vNAR domains as well as polynucleotide libraries for use in the generation of the inventive bi-specific vNARs. Furthermore, the invention provides pharmaceutical compositions comprising the inventive bispecific vNARs or fusion proteins comprising bi-specific vNAR domains for use in the treatment of pathological conditions in an individual. The invention also provides kits of parts comprising the bispecific vNAR domains or fusion proteins.