3′-OH unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing
US11001886B2 · kind B2 · utility
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27Claims
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Key dates
| Filing date | Jun 27, 2018 |
| Grant date | May 11, 2021 |
| Priority date | — |
| Expiry date | Dec 15, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/16
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
The present invention relates generally to 3′-OH unblocked nucleotides and nucleosides labeled and unlabeled with 5-methoxy-substituted nitrobenzyl-based photocleavable terminating groups for use in methods and systems related to DNA and RNA sequencing and analysis. These compounds may be used as reversible terminators as they exhibit fast nucleotide incorporation kinetics, single-base termination, high nucleotide selectivity, and rapid terminating group cleavage that results in a naturally occurring nucleotide.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.