Electrical detection process for peptides, proteins and other macromolecules
US11002657B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Jun 26, 2017 |
| Grant date | May 11, 2021 |
| Priority date | — |
| Expiry date | Nov 29, 2037 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/48721
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An aerolysin nanopore or a nanotube is used for the electrical detection of peptides, proteins separated by at least one amino acid and other macromolecules such as polysaccharides or synthetic or natural polymers present in a preparation where said nanopore or nanotube is inserted into a lipid membrane which is subjected to a difference in potential greater than −160 mV, in a reaction medium having an alkali metal halide electrolyte solution with a concentration of less than 6M and at a temperature of less than 40° C., and where said use is intended to differentiate said peptides, proteins and other molecules according to their length and their mass. Application to the sequencing of peptides and other molecules to differentiate them according to their length and mass with an amino acid-level or monomer-level resolution and to medical diagnosis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.