Determining the limit of detection of rare targets using digital PCR
US11004540B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 3, 2016 |
| Grant date | May 11, 2021 |
| Priority date | — |
| Expiry date | Jan 22, 2038 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B40/10
- WIPO fieldComputer technology
- WIPO sectorElectrical engineering
Abstract
A method for determining false positives calls in a biological data plot is provided. The method includes identifying a first data cluster as non-amplification data points within the biological data plot and identifying a second data cluster as wild-type positives within the biological data plot. The method further includes estimating a position in the biological data plot of a center of the first and second data clusters. The method further includes determining, for each data point within the first data cluster, a probability of belonging to the first data cluster and determining, for each data point within the second data cluster, a probability of belonging to the second data cluster. The method includes applying a probability threshold for each data point within the first and second data cluster to identify false positives.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.