Detecting residual host cell proteins in recombinant protein preparations

US11007849B2 · kind B2 · utility

Assignees

• Medlmmune, LLC · US
• The Regents of the University of California · US

Inventors

• Andrew Csordas · Lompoc, US
• James B. McGivney IV · Gaithersburg, US
• Hyongsok Soh · Lompoc, US
• Robert Strouse · Gaithersburg, US
• Faye Walker · Lompoc, US

Key dates

• Filing date: Aug 14, 2015
• Grant date: May 18, 2021
• Expiry date: May 28, 2037

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2310/16
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

This application provides a method of using aptamers to determine the presence of HCP's in a recombinant protein preparation, and provides methods of preparing a plurality of aptamers for use in the method of detecting a plurality of HCP's in a recombinant protein preparation. In accordance with the description, a method of determining the presence or absence of a plurality of HCP's in a recombinant protein preparation comprises: providing a recombinant protein preparation; providing a final pool of aptamers; combining the recombinant protein preparation with the final pool of aptamers; and determining the presence or absence of a plurality of HCP's.