Method for purifying and quantifying thrombin and its degradation polypeptides
US11008560B2 · kind B2 · utility
0Cited by
5References
10Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 15, 2018 |
| Grant date | May 18, 2021 |
| Priority date | — |
| Expiry date | Mar 15, 2038 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/96463
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Provided is a method for purifying α-thrombin and for quantifying α-thrombin and its degradation polypeptides in a liquid proteinatious solution. The method employs a one-step anion exchange chromatography method. The method allows purification and/or quantification of a homogenous post-translationally modified α-thrombin. The method can also be used for purification and/or quantification of β-thrombin.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.