Tagged nucleotides useful for nanopore detection

US11008613B2 · kind B2 · utility

Assignee

• Roche Sequencing Solutions, Inc. · US

Inventors

• Dmitriy Gremyachinskiy · San Francisco, US
• Peter Crisalli · Mountain View, US
• Andrew Trans · Bishop, US
• Ashwini Bhat · Milpitas, US

Key dates

• Filing date: Mar 20, 2020
• Grant date: May 18, 2021
• Expiry date: Mar 20, 2040

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6823
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

The present disclosure relates to compounds comprising a negatively-charged polymer moiety which is capable of entering a nanopore and upon entering a nanopore in the presence of positive ions results in an increased flow of the positive ions through the nanopore. The present disclosure provides methods of preparing the compounds and for their use as nanopore-detectable tags, in particular, for nanopore-based nucleic acid detection and sequencing.