Method for determining a concentration of a target analyte in a sample of bodily fluid

US11016098B2 · kind B2 · utility

Assignee

• Roche Diagnostics Operations, Inc. · US

Inventors

• Uwe Kobold · Wielenbach, DE
• Roland Thiele · Murnau am Staffelsee, DE
• Noah Weiss · Munich, DE
• Benjamin Tiemann · Munich, DE

Key dates

• Filing date: Oct 12, 2018
• Grant date: May 25, 2021
• Expiry date: Oct 12, 2038

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2458/15
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method and an apparatus for determining a concentration of a target analyte in a sample of bodily fluid are disclosed. The method involves providing a sample of bodily fluid including the target analyte, providing an internal standard solution including a mixture of components having a plurality of isotopes of the target analyte, wherein a concentration of each isotope is unknown, adding the internal standard solution to the sample, analyzing the sample including the internal standard solution using a mass spectrometer, creating a sample function curve based on signal intensities, wherein the signal intensities define arbitrary units, transferring an analyte signal into a corresponding arbitrary analyte unit by means of the sample function curve, and transferring the arbitrary analyte unit into the concentration of a target analyte by means of a standardization function representing a curve of concentrations depending on the arbitrary units.