Methods for quantitating individual antibodies from a mixture
US11020686B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 8, 2017 |
| Grant date | Jun 1, 2021 |
| Priority date | — |
| Expiry date | Jun 12, 2038 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2030/027
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present disclosure relates to, inter alia, a method of quantitating an amount of an antibody molecule from a mixture comprising two or more antibody molecules, comprising separating each of the two or more antibody molecules from the mixture by hydrophobic interaction chromatography high performance liquid chromatography (HIC-HPLC) and quantitating an amount of each antibody molecule, wherein the molecular weight of each antibody molecule is within 15 kDa of any other antibody molecule in the mixture and either each antibody molecule is different from another antibody molecule in the mixture by more than about 0.25 unit on the Kyte & Doolittle hydropathy scale or each of the antibody molecules when nm alone on HIC-HPLC elutes at distinct run time with little overlap from the other antibody molecules in the mixture, or both.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.