Devices and methods for sample analysis

US11022598B2 · kind B2 · utility

Assignee

• Abbott Laboratories · US

Inventors

• Jeffrey B. Huff · Carlsbad, US
• Mark A. Hayden · Ingleside, US
• Peter J. Karabatsos · Glencoe, US
• Andrew S. Schapals · Pleasant Prairie, US
• Anthony S. Muerhoff · Kenosha, US
• M. Felicia Bogdan · Gurnee, US
• Thomas P. Leary · Kenosha, US
• Shelley R. Holets-McCormack · Waukegan, US
• Sophie Laurenson · Basel, CH
• Andrew Fischer · Euless, US
• Richard Haack · Skokie, US
• Stefan Hershberger · Highland Park, US
• Dustin House · Carrollton, US
• Lei Qiao · Addison, US
• M. Shawn Murphy · Allen, US
• Mark R. Pope · Grayslake, US
• Edna Prieto-Ballengee · Dallas, US
• Qiaoqiao Ruan · Kildeer, US
• Pathik H. Soni · Chicago, US
• Sergey Y. Tetin · Moran Corners, US
• Lyle Yarnell · Richardson, US

Key dates

• Filing date: Oct 3, 2017
• Grant date: Jun 1, 2021
• Expiry date: Oct 3, 2037

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2458/10
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided.