Method for producing haploid, dihaploid and doubled haploid plants by isolated microspore culture
US11026381B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 26, 2016 |
| Grant date | Jun 8, 2021 |
| Priority date | — |
| Expiry date | Sep 27, 2037 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA01H6/34
- WIPO fieldFood chemistry
- WIPO sectorChemistry
Abstract
The present invention relates to a method for producing haploid, dihaploid, polyhaploid and/or doubled haploid plants of the family Cucurbitaceae from isolated microspores, wherein said method comprises a) culturing isolated microspores to obtain embryos competent for plant regeneration, wherein the microspores have been isolated from plant material of a donor plant of the family Cucurbitaceae; and b) regenerating plants from the embryos; wherein step (a) comprises contacting the microspores with one or more inhibitor of histone deacetylase (HDACi) and one or more polyamine. The present invention also relates to a method for producing haploid, dihaploid, polyhaploid and/or doubled haploid embryos, to related kits and compositions, and to plants obtained according to the methods.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.