Polypeptide assemblies and methods for the production thereof

US11028383B2 · kind B2 · utility

Assignees

• University of Washington · US
• University of Utah Research Foundation · US

Inventors

• Neil P. KING · Seattle, US
• Wesley Sundquist · Salt Lake City, US
• Joerg Votteler · Salt Lake City, US
• Yang HSIA · Seattle, US
• David Baker · Ann Arbor, US
• Jacob BALE · Seattle, US
• Marc Joseph LAJOIE · Seattle, US
• Gabriel Butterfield · Seattle, US
• Elizabeth Gray · Minneapolis, US
• Daniel Stetson · Stow, US

Key dates

• Filing date: Nov 6, 2019
• Grant date: Jun 8, 2021
• Expiry date: Nov 6, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/735
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The application discloses multimeric assemblies including multiple oligomeric substructures, where each oligomeric substructure includes multiple proteins that self-interact around at least one axis of rotational symmetry, where each protein includes one or more polypeptide-polypeptide interface (“O interface”); and one or more polypeptide domain that is capable of effecting membrane scission and release of an enveloped multimeric assembly from a cell by recruiting the ESCRT machinery to the site of budding by binding to one or more proteins in the eukaryotic ESCRT complex (“L domain”); and where the multimeric assembly includes one or more subunits comprising one or more polypeptide domain that is capable of interacting with a lipid bilayer (“M domain”), as well as membrane-enveloped versions of the multimeric assemblies.