Microorganisms and methods for producing 2-pyrone-4,6-dicarboxylic acid and other compounds
US11028418B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 19, 2020 |
| Grant date | Jun 8, 2021 |
| Priority date | — |
| Expiry date | Feb 19, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y301/01057
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Recombinant microorganisms configured for enhanced production of compounds such as 2-pyrone-4,6-dicarboxylic acid (PDC) and methods of using the recombinant microorganisms for the production of these compounds. The recombinant microorganisms include one or more modifications that reduce 2-pyrone-4,6-dicarboxylic acid (PDC) hydrolase activity, 4-carboxy-2-hydroxy-6-methoxy-6-oxohexa-2,4-dienoate (CHMOD) cis-trans isomerase activity, 4-carboxy-2-hydroxy-6-methoxy-6-oxohexa-2,4-dienoate (CHMOD) methyl esterase activity, and/or vanillate/3-O-methylgallate O-demethylase activity. The recombinant microorganisms can be used to generate PDC from media comprising plant-derived phenolics, such as syringyl phenolics, guaiacyl phenolics, and p-hydroxyphenyl phenolics. The plant-derived phenolics can be derived from pretreated lignin, including depolymerized lignin or other chemically altered lignin.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.