Real-time and quantitative measurement method for cell traction force
US11029285B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 28, 2017 |
| Grant date | Jun 8, 2021 |
| Priority date | — |
| Expiry date | Mar 5, 2038 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2291/02827
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A real time and quantitative method of measuring traction force of living cells include the following procedures. Place AT-cut and BT-cut quartz crystals of the same frequency, surface morphology and/or modified with the same cell adhesion molecules in petri dishes or detection cells; add the cells to the petri dishes or detection cells, the cell traction force at arbitrary time t during adhesion of the cells or under different internal/external environmental stimulations is estimated by the following equation: ΔSt=(KAT−KBT)−1[tqATΔftAT/frAT−tqBTΔftBT/frBT]. The method can be used to track the dynamic changes of cells generated force during the adhesion of cells and under different internal/external environmental stimulations, such as the effects of drugs. The drugs can be added before or after the adhesion of the cells. This method is suitable for all adherent cells, including primary cells and passage cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.