Polymerase-template complexes
US11034999B2 · kind B2 · utility
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22Claims
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Key dates
| Filing date | Jul 8, 2019 |
| Grant date | Jun 15, 2021 |
| Priority date | — |
| Expiry date | Jul 8, 2039 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y207/07007
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present disclosure provides methods and compositions for enhancing the processivity of a polymerase in catalyzing template-dependent DNA synthesis in high concentrations of salt. Also disclosed are methods and compositions for enhancing the assembly of polymerase-template complex compatible with active DNA synthesis in the presence of low levels of nucleotides and at a high temperature, such as temperatures at or near the melting temperature of the polymerase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.