Optimization methods for making a synthetic gene
US11041158B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Dec 21, 2015 |
| Grant date | Jun 22, 2021 |
| Priority date | — |
| Expiry date | Jul 14, 2038 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B30/20
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for making a synthetic gene are provided. The methods find use in optimizing a candidate gene nucleic acid sequence for expression in a selected target expression system. The method identifies stable or retained sequences in the candidate gene nucleic acid sequence, identifies disallowed sequences, develops a statistical model based on a whole genome, a partial genome, or transcriptome sequences of the target expression system, generates an optimized candidate gene nucleic acid sequence for use in the target expression system, and makes a synthetic gene comprising the optimized candidate gene nucleic acid sequence. The method allows for optimization of the candidate gene nucleic acid sequence without removing certain stable or retained sequences. Rather, the activity of these sites is positionally modulated or inactivated through upstream and downstream modifications of codons and/or sequence patterns.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.