Genetic markers for discrimination and detection of red sea bream iridovirus causing infectious aquatic organism diseases, and method of discriminating and detecting the virus using the same
US11041190B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 20, 2019 |
| Grant date | Jun 22, 2021 |
| Priority date | — |
| Expiry date | Oct 20, 2039 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2525/107
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Genetic markers are described for discriminating or detecting viruses causing infectious aquatic organism diseases, and a method of discriminating and detecting the viruses using the same is disclosed, in which the method includes selecting and amplifying a DNA nucleotide sequence encoding a gene specific for viral hemorrhagic septicemia virus (VHSV), red sea bream iridovirus (RSIV) or infectious spleen and kidney necrosis virus (ISKNV), which is a virus causing red sea bream iridovirus disease, or Koi herpesvirus (KHV); hybridizing a peptide nucleic acid (PNA) that specifically recognizes the amplification product; controlling the temperature of the hybridization product to obtain a temperature-dependent melting curve; and discriminating the viral type or detecting whether or not fish are infected with the viral type by analyzing the obtained melting curve to determine a melting temperature.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.