Systems and methods for next generation sequencing uniform probe design
US11041200B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Oct 21, 2020 |
| Grant date | Jun 22, 2021 |
| Priority date | — |
| Expiry date | Oct 21, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC40B40/06
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Systems and methods are provided for determining an optimized probe set. The method proceeds by obtaining a set of probes, where each probe has a respective concentration. The set of probes is assayed against a sample library, and at least i) a respective recovery rate for each probe in the set of probes, and ii) a median recovery rate for the set of probes are obtained. Modify the respective concentration of each probe that does not satisfy predetermined recovery rate threshold. Reevaluate the set of probes against the sample library. Repeat the modifying and reevaluation until the respective updated recovery rate for each probe in the updated set of probes satisfies the predetermined recovery rate threshold, thereby providing the optimized set of probes for the sample library.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.