Integrated platform for characterization of single cells or small cell clusters

US11045807B2 · kind B2 · utility

Assignee

• ARIZONA BOARD OF REGENTS FOR AND ON BEHALF OF ARIZONA STATE UNIVERSITY · US

Inventors

• Deirdre Meldrum · Phoenix, US
• Laimonas Kelbauskas · Gilbert, US
• Yanqing Tian · Tempe, US
• Honor Glenn · Mesa, US
• Clifford L. Anderson · Tempe, US
• Kristen Lee · Mesa, US
• Ganquan Song · Mesa, US
• Liqiang Zhang · Shandong, CN
• Jeff Houkal · Los Angeles, US
• Fengyu Su · Tempe, US
• Benjamin Ueberroth · Scottsdale, US
• Jacob Messner · Mesa, US
• Hong Wang · Cypress, US
• Kimberly Bussey · Scottsdale, US

Key dates

• Filing date: Feb 26, 2018
• Grant date: Jun 29, 2021
• Expiry date: Feb 26, 2038

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2015/1006
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

An integrated technological platform enabling real-time quantitative multiparameter metabolic profiling, utilizing either or both of extra and intracellular optical sensors, individually or simultaneously. A scalable embedded micropocket array structure, generally fabricated on fused silica substrates, facilitates the integration of multiple, spatially separated extracellular sensors for multiparameter analysis in a container formed with the use of an activation mechanism forming part of a device configured to hold the container during the measurements. The creation of hermetically sealed microchambers is carried out with a pneumatically and/or mechanically and/or electromechanically driven device that is “floating” within the holding device and that is optionally equipped with a vacuum/suction mechanism to hold a component of the container at its surface.