Matrix metalloprotease-cleavable and serine protease-cleavable substrates and methods of use thereof

US11046759B2 · kind B2 · utility

Assignee

• CytomX Therapeutics, Inc. · US

Inventors

• Stephen Moore · Las Vegas, US
• Margaret Thy Luu Nguyen · San Francisco, US
• Daniel Robert Hostetter · Palo Alto, US
• Olga Vasiljeva · Cupertino, US
• Jason Gary Sagert · Lompoc, US
• Jonathan Alexander Terrett · Slough, GB
• James William West · Lompoc, US

Key dates

• Filing date: Jan 20, 2016
• Grant date: Jun 29, 2021
• Expiry date: Jan 20, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/74
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The invention relates generally to polypeptides that include at least a first cleavable moiety (CM1) that is a substrate for at least one matrix metalloprotease (MMP) and at least a second cleavable moiety (CM2) that is a substrate for at least one serine protease (SP), to activatable antibodies and other larger molecules that include these polypeptides that include at least a CM1 that is a substrate for at least one MMP protease and at least a CM2 that is a substrate for at least one SP protease, and to methods of making and using these polypeptides that include at least a CM1 that is a substrate for at least one MMP protease and at least a CM2 that is a substrate for at least one SP protease in a variety of therapeutic, diagnostic and prophylactic indications.