Patent · US Active

Expression vectors with promoter and nucleic acid

US11046961B2 · kind B2 · utility

0Cited by

5References

11Claims

0Family size

Assignee

BASF SE

Inventors

Christian Degering · Erkrath, DE
Thorsten Eggert · Hattingen, DE
Stefan Evers · Mettmann, DE
Karl-Heinz Maurer · Erkrath, DE
Johannes Bongaerts · Dormagen, DE

Key dates

Filing date	Dec 2, 2019
Grant date	Jun 29, 2021
Priority date	—
Expiry date	Dec 2, 2039

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N15/75
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The aim of the invention is to improve the secretion of a protein from a host cell in order to increase the product yield of protein in a fermentation process. This is achieved by an expression vector comprising a) a promoter sequence and b) a nucleic acid sequence that codes for a protein. The protein comprises a signal peptide and an additional amino acid sequence, and the signal peptide comprises an amino acid sequence that is at least 80% identical to the amino acid sequence specified in SEQ ID NO: 2, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 4, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 6, or the signal peptide comprises an amino acid sequence that is structurally homologous to at least one of said sequences.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.