Patent · US Active

Lux expression in cells and methods of use

US11046962B2 · kind B2 · utility

0Cited by

7References

32Claims

0Family size

Inventors

Daniel Close · Knoxville, US
Steven Ripp · Knoxville, US
Gary S. Sayler · Strawberry Plains, US
Michael Conway · Madison, US

Key dates

Filing date	May 29, 2020
Grant date	Jun 29, 2021
Priority date	—
Expiry date	May 29, 2040

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Y114/14003
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

Cells, including stem cells, comprising an autobioluminescent phenotype, wherein the cells emit a luminescent signal in the absence of an exogenous luminescent stimulator, are provided. The luminescent signal may be constitutive, inducible, repressible, or tissue-specific. The cells express a synthetically engineered bacterial luciferase (lux) cassette, i.e., the luxCDABEfrp gene cassette. The cells may comprise luxA, luxB, luxC, luxD, luxE, and flavin reductase. The cells may each express a combined expression level of luxC, luxD, luxE, and flavin reductase that is from ten to forty times greater than a combined expression level of luxA and luxB. Further, methods of making and using the cells comprising an autobioluminescent phenotype are disclosed herein.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.