Bicistronic expression vector for antibody expression and method for producing antibody using same
US11046975B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 7, 2014 |
| Grant date | Jun 29, 2021 |
| Priority date | — |
| Expiry date | Oct 7, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2840/203
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a bicistronic expression vector for antibody expression, an animal cell transfected with the expression vector, and a method for producing an antibody including culturing the animal cell, in which the expression vector includes a first expression cassette including ‘promoter-UTR-intron-antibody light chain gene-polyA’ and a second expression cassette including ‘promoter-UTR-intron-antibody heavy chain gene-internal ribosome entry site (IRES)-amplification gene-polyA’. An expression vector capable of expressing a desired antibody with high efficiency can be constructed using the bicistronic expression vector including an intron for antibody expression according to the present invention, and the expression vector can produce the antibody by culturing the transfected animal cell with stability and high efficiency.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.