Glucose dehydrogenase having modified electron transfer properties, and glucose measurement method
US11046993B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 31, 2016 |
| Grant date | Jun 29, 2021 |
| Priority date | — |
| Expiry date | Feb 1, 2037 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/66
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A glucose dehydrogenase having modified electron transfer properties, and a glucose measurement method and measuring kit using the glucose dehydrogenase are provided. Provided are a glucose dehydrogenase having at least 1, 2 or 3 amino acid substitutions, for example, amino acid substitution with a polar amino acid or alanine, in the region corresponding to the 457th to 477th position in SEQ ID NO: 1 of a glucose dehydrogenase having homology with SEQ ID NO: 1, a glucose measurement method, a measurement reagent kit and a sensor using the glucose dehydrogenase. The electron transfer properties of the glucose dehydrogenase of the present invention is modified and can be used in measuring glucose in the presence of a mediator with reduced concentration or in the absence of a mediator, and can be used, for example, in continuous glucose measurement.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.