Gradient coatings of biopeptides that promote endothelial cells selective adhesion and directional migration and methods of using the same
US11052176B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 10, 2017 |
| Grant date | Jul 6, 2021 |
| Priority date | — |
| Expiry date | Oct 10, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2539/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A two-layer gradient coating article is provided that is operable to cause selective adhesion and directional migration of endothelial cells. The first layer includes cell-resisting polymers that repels cells, the second layer includes one layer of peptides that has affinity to and binds specifically to endothelial cells. Furthermore, the peptides are distributed in a gradient, in which attached ECs migrate towards the direction of increased concentration, thus enriching the ECs to a desired locus. The combination of a cell-repelling layer and a graded affinity peptide produces a unique result of selective adhesion, directional migration, thus local enrichment of endothelial cells. A method for using such gradient coating article and its potential use in treating cardiovascular diseases are also provided. The invention provides an inexpensive, stable and effective means for attracting ECs to desirable locations.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.