Patent · US Active

Method for separating virus-like particles from a cell suspension

US11053478B2 · kind B2 · utility

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19Claims

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Assignee

ARTES BIOTECHNOLOGY GMBH · DE

Inventors

David B. Wetzel · Laguna Beach, US
Volker Jenzelewski · Neuss, DE
Michael Piontek · Velbert, DE

Key dates

Filing date	Jun 11, 2019
Grant date	Jul 6, 2021
Priority date	—
Expiry date	Jan 29, 2040

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2730/10151
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A method for separating virus-like particles from a cell suspension of host cells. The virus-like particles having at least one envelope protein embedded in a lipid double membrane including at least a portion corresponding to a small envelope protein of a virus of the family Hepadnaviridae. The host cells are disrupted to obtain a first suspension. A supernatant containing the virus-like particles is separated from the first suspension. Then, an adsorbent is added to the supernatant and separated off. Then, the virus-like particles are desorbed from the adsorbent by adding a desorption buffer. A soluble calcium salt is added to a supernatant separated from the second suspension to form a precipitate, the precipitate formed is separated off and transferred to a third suspension. The virus-like particles are separated from the third suspension and purified.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.