Iterative genome editing in microbes
US11053506B2 · kind B2 · utility
1Cited by
31References
27Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 20, 2020 |
| Grant date | Jul 6, 2021 |
| Priority date | — |
| Expiry date | Mar 20, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2800/80
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to methods for editing the genome of a microbial host cell in successive rounds of transformation. The method allows the introduction of genetic edits into the genome of a microbial host cell in an iterative fashion that does not require the use of functional counterselection following at least one round of transformation. It can be used to rapidly stack genetic edits in the genome of a microbial host cell. Kits for performing the methods are also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.