Systems and methods for sequencing in emulsion based microfluidics
US11053541B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Sep 8, 2017 |
| Grant date | Jul 6, 2021 |
| Priority date | — |
| Expiry date | May 7, 2040 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B20/00
- WIPO fieldComputer technology
- WIPO sectorElectrical engineering
Abstract
Methods, libraries, and kits for nucleotide sequencing are provided. For example, methods can comprise providing a plurality of partitions, the partitions comprising a set of two or more different primers and a target nucleic acid, wherein different partitions contain different sets of primers with a majority of primers in the sets being common with a different primer set but wherein any two different partitions having a common primer contain no more than one common primer; and hybridizing the target nucleic acid to the primers in the reaction partitions under conditions in which fully complementary primers hybridize to the target sequence and primers that are not fully complementary do not hybridize to the target nucleic acid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.