Methods for estimating cluster numbers
US11060130B2 · kind B2 · utility
2Cited by
11References
14Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 11, 2015 |
| Grant date | Jul 13, 2021 |
| Priority date | — |
| Expiry date | Sep 24, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6874
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to methods of imaging template hybridisation for estimating cluster numbers prior to solid phase amplification and sequencing. More particularly, an initial round of imaging is carried out at the single molecule template hybridisation stage which allows a general estimation of cluster numbers prior to clusters being formed. Amplification of the signal allows single molecule imaging to be carried out using standard sequencing imaging apparatus.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.