Droplet microfluidic device and methods of sensing the results of an assay therein
US11061015B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 28, 2015 |
| Grant date | Jul 13, 2021 |
| Priority date | — |
| Expiry date | Jan 12, 2037 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2030/8813
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of determining the result of an assay in a microfluidic device includes the steps of: dispensing a sample droplet onto a first portion of an electrode array of the microfluidic device; dispensing a reagent droplet onto a second portion of the electrode array of the microfluidic device; controlling actuation voltages applied to the electrode array to mix the sample droplet and the reagent droplet into a product droplet; sensing a dynamic property of the product droplet; and determining an assay of the sample droplet based on the sensed dynamic property. The dynamic property is a physical property of the product droplet that influences a transport property of the product droplet on the electrode array. Example dynamic properties of the product droplet include the moveable state, split-able state, and viscosity based on droplet properties. The method may be used to perform an amoebocyte lysate (LAL) assay.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.