Method of transforming bacterial cells

US11066653B2 · kind B2 · utility

Assignee

• BASF SE

Inventors

• Max Fabian Felle · Ludwigshafen, DE
• Stefan Seemayer · Ludwigshafen, DE

Key dates

• Filing date: Jul 11, 2018
• Grant date: Jul 20, 2021
• Expiry date: Jul 11, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/75
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention is directed to a producing a DNA methyltransferase in a recombinant host cell, wherein the DNA methyltransferase methylates DNA resulting in a DNA containing 5-methylcytosine within the recognition sequence GCNGC and wherein the DNA methyltransferase comprises less than (35) amino acid residues between amino acid residue (72) and amino acid residue (106) according to the numbering of SEQ ID NO: 33. Furthermore, the present invention is directed to the use of such DNA methyltransferase for the production of bacterial transformants comprising the steps of (a) introducing into a first bacterial host cell a polynucleotide comprising a polynucleotide sequence encoding the DNA methyltransferase to produce a methylated DNA and (b) transferring the methylated DNA from the first bacterial host cell into a second bacterial host cell, wherein the second bacterial host cell comprises a restriction endonuclease able to degrade the DNA but unable to degrade the methylated DNA.