Multiplexed deterministic assembly of DNA libraries
US11066663B2 · kind B2 · utility
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36References
23Claims
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Key dates
| Filing date | Oct 31, 2019 |
| Grant date | Jul 20, 2021 |
| Priority date | — |
| Expiry date | Oct 31, 2039 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC40B50/06
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present disclosure relates to methods of joining three or more double-stranded (ds) or single-stranded (ss) DNA molecules of interest in vitro or in vivo. The method allows the joining of a large number of DNA fragments, in a deterministic fashion. It can be used to rapidly generate nucleic acid libraries that can be subsequently used in a variety of applications that include, for example, genome editing and pathway assembly. Kits for performing the method are also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.