Construct for epigenetic modification and its use in the silencing of genes

US11072782B2 · kind B2 · utility

Assignee

• Albert-Ludwigs-Universitaet Freiburg · DE

Inventors

• Toni CATHOMEN · Freiburg (Elbe), DE
• Claudio MUSSOLINO · Glückstadt, DE
• Tatjana Cornu · Freiburg (Elbe), DE
• Tafadzwa Mlambo · Zürich, CH
• Sandra NITSCH · Glückstadt, DE
• Jamal ALZUBI · Freiburg (Elbe), DE
• Marianna ROMITO · Freiburg (Elbe), DE

Key dates

• Filing date: Apr 11, 2018
• Grant date: Jul 27, 2021
• Expiry date: May 17, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/80
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Herein described is a construct for epigenomic modification of genes composed of: (a) a Krüppel-associated box zinc finger protein or homologous, (b) a DNA region capable of binding to the target gene or homologous, (c) a human DNA methyltransferase DNMT3A or homologous and (d) a murine DNA methyltransferase Dnmt3L or homologous, wherein components a), b), c) and d) are linked to each other either directly or via at least one linker. The construct is a designer epigenome modifier that can be used to silence genes coding for a protein in leukocytes that avoids the internalization of HI viruses in immune cells.