Method for preparing nanohybrid used for ratiometric fluorescence and ratiometric electrochemical sensing simultaneously
US11073517B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 3, 2019 |
| Grant date | Jul 27, 2021 |
| Priority date | — |
| Expiry date | Apr 3, 2039 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2400/50
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for preparing a nanohybrid used for ratiometric fluorescence and ratiometric electrochemical sensing simultaneously is provided. Surface-aminated (—NH2) SiO2 nanospheres encapsulating an electroactive material A or B are prepared and conjugated with surface-carboxylated (—COOH) carbon dots (CDs) or gold nanoclusters (AuNCs) to prepare a conjugate, and the conjugate is conjugated with a DNA aptamer terminated with —NH2. Ions or biomolecules are added to two types of DNA-conjugate dispersions, and ratiometric florescence sensing is realized by fitting the linear relationship between ratiometric fluorescent peak intensity ICDs/IAuNCs and a specific ion concentration or a specific biomolecule concentration. A-SiO2@CDs-DNA is attached to the surface of a gold electrode based on a DNA terminal —SH and Au—S bonding; B—SiO2@AuNCs-DNA and ions or biomolecules are added, and ratiometric electrochemical sensing is realized by fitting the linear relationship between the specific ion concentration or the specific biomolecule concentration and the ratiometric current peak intensity IB/IA.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.