Synthetic compound for improving efficiency of transfection

US11077205B2 · kind B2 · utility

Assignee

• Klinikum der Universität München · DE

Inventors

• Shan Guan · Fremont, US
• Joseph Rosenecker · Schkeuditz, DE

Key dates

• Filing date: Mar 30, 2017
• Grant date: Aug 3, 2021
• Expiry date: Mar 30, 2037

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/85
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The invention concerns a synthetic compound for enabling transfection of eukaryotic cells by means of an amphiphilic block copolymer which synthetic compound comprises a peptide residue having at least one targeting sequence and a nucleic acid binding sequence which nucleic acid binding sequence comprises or consists of at least four consecutive amino acid residues which are positively charged at pH 7.4, wherein the targeting sequence is any sequence that causes a direction of the sequence to a position or a structure inside or on the surface of eukaryotic cells, characterized in that the synthetic compound further comprises a hydrophobic moiety covalently linked to the peptide residue, wherein the hydrophobic moiety comprises or consists of lipoic acid residue, lipoamide residue, a tetradecyl residue, a cholesteryl residue, or a further peptide residue having a sequence with more than 40% amino acid residues with hydrophobic side chains.