Methods for processing droplets containing biological entities
US11077441B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 3, 2016 |
| Grant date | Aug 3, 2021 |
| Priority date | — |
| Expiry date | Jul 13, 2037 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2035/1034
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Instruments and cartridges for processing droplets in emulsions containing biological entities such as cells. A method of such processing comprises providing a plurality of the entities in a fluid; preparing a droplet from the fluid; determining whether the droplet contains one or more entities of said plurality of entities, or whether said droplet does not contain a said entity; sorting said droplet dependent on an outcome of the determination; and dispensing the sorted droplet into a reservoir. The dispensing may comprise identifying and extracting the sorted droplet from a first fluidic flow path of said fluid by transferring the sorted droplet from into a second fluidic flow path and then ejecting the sorted droplet into a reservoir by applying pressure to the second fluidic flow path. The droplet contents may be tracked so that the contents of an individual droplet can be sorted, selectively dispensed, and retrieved.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.