Detection of target nucleic acid sequence by PTO cleavage and extension-dependent cleavage
US11078525B2 · kind B2 · utility
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8Claims
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Key dates
| Filing date | Mar 29, 2012 |
| Grant date | Aug 3, 2021 |
| Priority date | — |
| Expiry date | May 12, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6853
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to the detection of a target nucleic acid sequence by a PCEC (PTO Cleavage and Extension-Dependent Cleavage) assay. The present invention is characterized by generating a cleavage site for a nucleolytic enzyme on the extended duplex of which the formation is dependent on the presence of a target nucleic acid sequence. The present invention detects the occurrence of the cleavage of the extended duplex, thereby determining the presence of the target nucleic acid sequence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.