Nanopore coating for sensing chemical bond formation
US11079377B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 24, 2018 |
| Grant date | Aug 3, 2021 |
| Priority date | — |
| Expiry date | Sep 28, 2038 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2300/161
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A process for monitoring binding events, a coating compound, and a device for monitoring binding events are disclosed. The process includes providing two reservoirs that contain an electrically conductive fluid, wherein the reservoirs are separated by a membrane having a nanopore. The process also includes selecting a target compound, selecting a ligand based on the selection of the target compound, and preparing a coating that includes the coating compound, wherein the coating compound includes the ligand and a moiety that immobilizes the ligand. Additionally, the process includes applying the coating to the inner surface of the nanopore, adding the target compound to a conductive liquid in the first chamber, establishing a voltage gradient across the membrane, and electrically monitoring translocation of the target molecule. The device includes the reservoirs and the membrane having the coated nanopore.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.