Microscopy system and microscopy method for quantifying a fluorescence

US11079587B2 · kind B2 · utility

Assignee

• Carl Zeiss Meditec AG · DE

Inventors

• Alois Regensburger · Erlangen, DE
• Christoph Hauger · Aalen, DE
• Susanne Kohlhammer · Blaustein, DE
• Jonathan Essig · Neresheim, DE

Key dates

• Filing date: Mar 2, 2018
• Grant date: Aug 3, 2021
• Expiry date: May 6, 2039

Classification

• Technology area (CPC G): Physics
• CPC primary: G02B21/361
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A microscopy method for quantifying a fluorescence of protoporphyrin IX includes: imaging an object region onto a first detector field and a second detector field, wherein a first optical filter and a second optical filter, respectively, are arranged in the beam paths between the object region and the detector fields, the first optical filter and second optical filter respectively having a wavelength-dependent transmission characteristic; exciting at least a first fluorescence of protoporphyrin IX and a second fluorescence; recording first images and second images; and determining a spatially dependent fluorescence intensity of the first fluorescence in the object region by virtue of determining values representing a fluorescence intensity at locations in the object region, wherein the values are determined on the basis of the radiation intensities of the two detector fields detected in a spatially dependent manner and the spatially dependent wavelength-dependent detection efficiencies of the two detector fields.