Blood sample assay method

US11085066B2 · kind B2 · utility

Assignee

• AXIS-SHIELD AS · NO

Inventors

• Arne Ludvig Faaren · Oslo, NO
• Frank Frantzen · Oslo, NO
• Arne Kristian Nordhei · Oslo, NO
• Erling Sundrehagen · Oslo, NO
• Lars Ørning · Oslo, NO

Key dates

• Filing date: Oct 31, 2019
• Grant date: Aug 10, 2021
• Expiry date: Oct 31, 2039

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/92
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention provides an enzymatic method for measuring the concentration of one or more analytes in the plasma portion of a blood derived sample, containing a first and a second component, where said second component interferes with the measurement of said first component. The method includes: i) diluting the sample with a reagent mixture; ii) substantially removing blood cells; iii) using a reagent which serves to temporarily prevent reaction of the second component, to generate a blocked second component; iv) causing the selective reaction of a constituent of each analyte to directly or indirectly generate detectable reaction products, where one of the analytes is the first component; v) monitoring the detectable reaction product or products; vi) relating an amount of the detectable product or products and/or a rate of formation of the detectable product or products to the concentration of each analyte, where the concentration of at least the first component is related to a corresponding detectable reaction product by means of estimating an un-measurable (fictive) endpoint. Step iii) may be carried out at any stage up to and including step iv) but before steps v) or vi). The re…